PerlPrimer 1.1.14

Company: perlprimer.sourceforge.net

Date Added: May 10, 2013 | Visits: 405

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PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design.PerlPrimer's current features include the following: - Calculation of possible primer-dimers - Retrieval of genomic or cdna sequences from Ensembl(including both sequences automatically for QPCR) - Ability to BLAST search primers using the NCBI server or a local server - Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms - Ability to add cloning sequences to primers, automatically adjusted to be in-frame - QPCR primer design without manual intron-exon boundary entry PerlPrimer calculates primer melting temperature using J. SantaLucia's extensive nearest-neighbour thermodynamic parameters. It allows the user to specify the concentration of Mg2 , dNTPs and primers, or use standard PCR conditions. The result is a highly accurate prediction of primer melting temperature, giving rise to a maximum yeild of product when amplified.

Requirements: No special requirements

Platforms: *nix, Linux, Perl , BSD

Keyword: Bio, Bio Informatics Software, Bio Informatics Tool, Bio Informatics Utility, Informatics, Software

Users rating: 0/10

License: Freeware

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