Primers

The FastPCR software was developed to be an integrated tools environment that provides comprehensive facilities for designing primers for most PCR applications including standard, long distance, inverse, real-time, multiplex, unique and group-specific; overlap extension PCR (OE-PCR)...

JMotifFinder is a small, simple, easy to use application designed to offer users a graphical utility to find DNA Motifs such as Primers in huge EMBL Files. If you load an EMBL file containing exon and mRNA information into JMotifFinder, it will graphically display the location of the exons and...

Primer Design is a simple utility created in order to design DNA primers for mutagenesis in the BGME lab (JHU). It allows users to select or enter background sequence and add current and new mutations. During the design process, users are presented with various updating checks to guide them.


Platforms: Windows

An automatically-updating library of genomic and genetic data; that provides a fully integrated, visually-advanced toolset for - Sequence alignment and phylogenetics - Sequence analysis, Contig assembly, - primers, restriction analysis - BLAST. Protein structure viewing, NCBI, UniProt, Pubmed...


Platforms: Mac

Primrose is a simple, Java based application designed to help you identify potentially useful oligonucleotides for use as probes or PCR primers as phylogenetic tools.


Platforms: Windows

MIDesigner (MID Intelligent Designer) is a utility to design multiplex identifier (MID) primers for use in sequencing projects. Thus, it is similar in intent to Barcrawl.


Platforms: Windows, Mac, Linux

PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design.PerlPrimer's current features include the following: - Calculation of...


Platforms: *nix, Perl, BSD

RAPD Primer Design from Metagenomes (RPD-M) scans metagenome sequences identifying and determining relative frequency of candidate primers for Random Amplification of Polymorphic DNA (RAPD) assays.


Platforms: Mac, BSD, Linux

Design primers based on length, melting temperature, GC content, hairpins, dimerization, nucleotide repeats, and 3' GC clamp criteria.Import data from files or from Genbank.


Platforms: Matlab

msatcommander is a python program written to locate microsatellite (SSR, VNTR, &c) repeats within fasta-formatted sequence or consensus files. msatcommander will search for all di-, tri-, tetra-, penta-, and hexa-nucleotide repeats (with options to search for fewer repeat types and combinations...


Platforms: Mac

Gene Studio is a free tool for modelling of DNA manipulation. It will allow to work with vectors, to design primers, to clone molecules and provide user friendly intewrface.


Platforms: Mac

analyzes different features of multiple primers simultaneously, the melting temperature calculation for standard and degenerate oligonucleotides, GC content, primer PCR efficiency; primers are analyzed for all primer secondary structures including G-quadruplexes detection, hairpins, self-dimers...


Platforms: Mac

IOSN Live CD is an bootable CD that boots into a usable and fully featured Linux Desktop. In addition to standard FOSS productivity and multimedia applications, it is bundled with a User Guide to Using the Linux Desktop in print and multimedia formats as well as several IOSN Free/Open Source...


Platforms: *nix

IOSN Live CD is an bootable CD that boots into a usable and fully featured Linux Desktop. In addition to standard FOSS productivity and multimedia applications, it is bundled with a User Guide to Using the Linux Desktop in print and multimedia formats as well as several IOSN Free/Open Source...


Platforms: *nix

OLIGO is a multi-functional program that searches for and selects oligonucleotides from a sequence file for polymerase chain reaction (PCR), DNA sequencing, site-directed mutagenesis, and various hybridization applications. It calculates hybridization temperature and secondary structure of...


Platforms: Mac

The goal of BioX is to bring easy-to-use sequence analysis to Mac-using biologists. It features graphical editors for sequences and multiple alignments, and supports opening and saving of many different sequence file formats. Analysis functions currently include searching for primers, restriction...


Platforms: Mac

AlleleID is a comprehensive desktop tool designed to address the challenges of pathogen detection, bacterial identification or taxa/species discrimination using microarrays and TaqMan® and molecular beacon qPCR assays. With ClustalW multiple sequence alignment at its core, AlleleID can design...


Platforms: Mac

Geneious Pro is an integrated, cross-platform bioinformatics software suite for manipulating, finding, sharing, and exploring biological data such as DNA sequences or proteins, phylogenies, 3D structure information, publications, etc. It features sequence alignment and phylogenetic analysis,...


Platforms: Windows

Geneious Pro is an integrated, cross-platform bioinformatics software suite for manipulating, finding, sharing, and exploring biological data such as DNA sequences or proteins, phylogenies, 3D structure information, publications, etc. It features sequence alignment and phylogenetic analysis,...


Platforms: Windows

CNVision is a small, simple application designed to simplify CNV analysis of Illumina genotyping data. It will simplify all stages of predicting and analyzing CNVs, from running prediction algorithms and combining their results to visualizing the raw data and designing qPCR primers for...


Platforms: Windows