Pintail 1.1

Pintail is a tool for identifying anomalies, including chimeras, within 16S rDNA sequences. The program works by comparing evolutionary distances between a query and subject sequence over the length of the 16S rRNA gene (small subunit rRNA), by employing a sampling window of specified size, progressing a fixed number of bases at a time along the length of the gene.<br />Licence<br /><br />Pintail is open source software and is released under the terms of the GNU General Public Licence.<br />A quick guide to using Pintail<br /><br /> 1. Enter the 16S rRNA gene sequence to be investigated into the text box marked 'query sequence'.<br /> 2. Enter a reliable 16S rRNA gene sequence in the text box labelled 'subject sequence'.<br /> 3. Click 'Run' to carry out the analysis.<br /><br />A chimera is indicated if the plotted observed percentage differences line deviates significantly from the expected percentage difference line. This deviation is quantified with the Deviation from Expectation (DE) statistic. The program determines whether the calculated DE value is likely to be indicative of a chimera (or other sequence anomaly) and provides an assessment accordingly.

Requirements:	No special requirements
Platforms:	Mac, Mac OSX
Keyword:	Carry,  Chimera,  Click,  Deviates,  Differences,  Enter,  Labelled,  Length,  Observed,  Percentage,  Pintail,  Plotted,  Program,  Query,  Reliable,  Run,  Sequence,  Subject
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