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A Bayesian Adaptive Basis Algorithm for Single Particle Reconstruction 1.0

  Date Added: August 26, 2013  |  Visits: 221

A Bayesian Adaptive Basis Algorithm for Single Particle Reconstruction

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3D reconstruction algorithm for electron cryo-microscopyTraditional single particle reconstruction methods use either the Fourier or the delta function basis to represent the particle density map. We propose a more flexible algorithm that adaptively chooses the basis based on the data. Because the basis adapts to the data, the reconstruction resolution and signal-to-noise ratio (SNR) is improved compared to a reconstruction with a fixed basis. Moreover, the algorithm automatically masks the particle, thereby separating it from the background. This eliminates the need for ad-hoc filtering or masking in the refinement loop. The algorithm is formulated in a Bayesian maximum-a-posteriori framework and uses an efficient optimization algorithm for the maximization. Evaluations using simulated and actual cryogenic electron microscopy data show resolution and SNR improvements as well as the effective masking of particle from background.

Requirements: No special requirements
Platforms: Matlab
Keyword: Adhoc Automatically Background Eliminates Filtering Formulated Masking Masks Refinement
Users rating: 0/10

License: Shareware Size: 7.39 MB
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A BAYESIAN ADAPTIVE BASIS ALGORITHM FOR SINGLE PARTICLE RECONSTRUCTION RELATED
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