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unfoldprotein.m 1.0

  Date Added: April 16, 2013  |  Visits: 213

unfoldprotein.m

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UNFOLDPROTEIN(data, bracket) calculates the melting temperature, Tm, of a protein from a given Nx2 array of unfolding data (with N >= 10). In the case of unfolding studies performed using circular dichroism spectroscopy, the second Tm calculated using the maximum of the first derivative of the CD signal as a function of temperature should be used in order to minimize errors due to baseline correction. The midpoint method is the more classical way to calculate Tm, but it is susceptible to non-ideal baseline measurements. Van't Hoff analysis is unreliable in the case of CD, and should only be considered reliable in the context of differential scanning calorimetry experiments. For more information, see

Requirements: No special requirements
Platforms: Matlab
Keyword: Baselines Calorimetry Considered Context Differential Enthalpies Experiments Files Gttwo Information Ltjohn Protein Reliable Scanning Unreliable Van Weeks White
Users rating: 0/10

License: Freeware Size: 10 KB
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